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Following incubation with primary antibodies and rinsing, the blots were incubated for 1 h at room temperature with HRP-conjugated secondary antibody, according to the manufacturers recommended ratio

The gut: satiety signaling

Semaglutide offers a promising metabolic approach for obesity-related HFpEF by improving patient-reported outcomes (KCCQ score, physical function), which are increasingly prioritized in HFpEF management

These medications work by mimicking hormonesGLP-1 for semaglutide, GLP-1 and GIP for tirzepatidethat help regulate blood sugar levels and appetite, leading to improved metabolic function and subsequent weight reduction