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Per-component breakdown (fixed ratio)

Cell lines and culture conditions HepG2 cells, a human hepatoma cell line, were obtained from the American Type Culture Collection (Manassas, VA) and were cultured in Dulbeccos modified Eagles medium (DMEM) containing 10% (v/v) fetal bovine serum, 2 mM glutamine, 5 U/mL penicillin, and 50 g/mL streptomycin at 37 C in a humidified O 2 /CO 2 (19:1) atmosphere

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Additionally, cAMP/PKA signaling rapidly mobilizes GH-containing secretory granules and promotes their fusion with the plasma membrane, causing acute release of stored GH into the hypophyseal capillaries that drain into systemic circulation

Then I go out by decade: at 60, 70, 80, 90