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The two reconstitution ratios most often described in community protocols are below

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The mechanistic basis involves GHK-CuCuSOD1 (cytoplasmic Cu/Zn-SOD) enhanced activity (NBT reduction assay: +31 5%), reducing HO-driven JNK activation via ASK1-Thr845 phosphorylation (ThioredoxinReductase-Trx1 redox buffer maintained)

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