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PBS (177 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 5.5 was used as dilution and running buffer, while PBS (195 mM phosphate, 85 mM NaCl, 0.05% (v/v) Tween 20) at pH 7.4 was used as regeneration buffer

The SECIS selenocysteine (sec) insertion sequence represents mRNA and serves as a platform for the recruitment of Sec -ARNt translation stretching factors that decode the UGA codon for the incorporation of Se into selenoproteins [43]