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Quantitative real-time PCR Total RNA from INS-1E cells and the INS-1E-derived 2A -adrenergic receptor knockout cell line were isolated using the RNeasy Plus micro Kit (Qiagen, Valencia, CA)

Short-term effects of alcohol consumption The short-term effects of alcohol consumption range from a decrease in anxiety and motor skills and euphoria at lower doses to intoxication (drunkenness), to stupor, unconsciousness, anterograde amnesia (memory "blackouts"), and central nervous system depression at higher doses

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