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NP-40 (600 l, 0.1% in PBS) was added to each sample and transferred to centrifugation columns (Fisher Scientific Pierce) to perform washing by gravity flow
Behavioral analysis following ethanol administration The Laboratory Animal Behaviour Observation, Registration, and Analysis System (LABORAS, Metris B.V., Hoofddorp, Netherlands) was used to investigate rat locomotor movements, following the protocols previously outlined by Castagne et al
Curr Drug Abuse Rev
RNA sequencing: the teenage years
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