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Human embryonic kidney cells 293 (HEK293), Madin Darby canine kidney cells (MDCK), and B 16F10 murine melanoma cells were grown in DMEM, 10% fetal bovine serum (FBS)

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Elevated circulating IGF-1 inhibits GHRH secretion and directly suppresses somatotroph GH release, reducing endogenous IGF-1 synthesis for the duration of IGF-1 LR3 administration

These therapies hold promise as nonspecific immunomodulators, offering new approaches to regulating gut microbiota composition and potentially predicting patient responses to treatment